Biological Indicator for Steam Sterilization, G. Stearothermophilus Spores, 50 Vials, 24-Hour Readout

The Geobacillus stearothermophilus biological indicator is designed for monitoring steam sterilization cycles in autoclaves operating between 121°C and 134°C. Each self-contained unit includes a filter paper spore carrier, an internal glass ampoule containing culture medium, and an outer polypropylene tube. The result is determined by observing the culture medium color after incubation.

Features
• Product type: self-contained biological indicator for steam sterilization.
• Test organism: Geobacillus stearothermophilus.
• Spore population: minimum 10⁶.
• Sterilization temperature: 121°C to 134°C.
• Incubation temperature: 55°C to 60°C.
• Final readout: 24 hours.
• Culture medium volume: 0.5 to 0.7 mL.
• Materials: polypropylene, glass, and filter paper.
• Package quantity: 50 single-use vials.

Result Interpretation
• Purple or violet: no detectable bacterial growth under the test conditions.
• Yellow: positive result indicating bacterial growth and requiring evaluation of the sterilization process.

Instructions for Use
1. Place the indicator in a suitable test pack and position it in the autoclave according to the equipment instructions.
2. Run the sterilization cycle and allow the indicator to cool for at least 10 minutes.
3. Activate the indicator using the designated mechanism to crush the internal glass ampoule.
4. Incubate at 55°C to 60°C for 24 hours. Begin incubation within two hours after the cycle ends.
5. Examine the culture medium color and record the result.

Storage and Safety
• Store in a dry environment at 15°C to 30°C, away from direct sunlight and chemical sterilizing agents.
• Wear protective eyewear and suitable gloves when handling the indicators.
• Allow the indicator to cool before activation and avoid squeezing the plastic tube directly with your fingers.
• Positive indicators must be treated before disposal according to the product instructions and applicable local medical waste regulations.

Package Contents
• 50 self-contained biological indicators containing Geobacillus stearothermophilus spores, a filter paper carrier, and an internal glass ampoule with culture medium.

This product is intended for biological monitoring of steam sterilization processes, with visual result interpretation after incubation under the specified conditions.